General Cell Based Assays
Meso Scale Discovery RPLEX HUM Nucleotidase ASY
The R-Plex Human 5 -Nucleotidase Assay from Meso Scale Diagnostics measures 5 -Nucleotidase in human serum plasma and cell culture supernatants using electrochemiluminescence (ECL) technology It offers high sensitivity a wide dynamic range and low sample volume requirements This assay quantifies 5 -Nucleotidase a glycoprotein in purine metabolism aiding in studying liver disease cancers and gout With a testing time of 3 5 hours it enables efficient analysis of 5 -Nucleotidase levels
Eurofins Discoverx PathHunter Human SCTR β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human SCTR β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure SCTR (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human S1P3 (EDG3) β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human S1P3 (EDG3) β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure EDG3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical ThIol DetectIon asy KIt 480Wel
The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 10pl Kit. (93-0838E3CP0L)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR171 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0415E2ACP1L)
The PathHunter GPR171 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR171 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR171 and EA-tagged β-Arrestin-2. Activation of GPR171 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HCK Kinase Binding Assay, 10pl Kit. (96-0041E15CP0L)
The InCELL Hunter eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IFNGR1/IFNGR2 Dimerization Assay, 10pl Kit. (93-1098E3CP5L)
The PathHunter IFNGR1/IFNGR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IFN-g) induced dimerization of IFNGR1/IFNGR2 receptors. This product uses EFC technology. The cell line included overexpresses IFNGR1 target protein fused to PK alongside IFNGR2 target protein fused to EA. Upon ligand-induced dimerization of IFNGR1/IFNGR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biotium ALDH1A1 (Aldehyde Dehydrogenase 1A1) (ALDH1A1/1381), CF568 conjugate, 0.1mg/mL
Aldehyde dehydrogenase 1 family member A1 (ALDH1A1), also known as retinal dehydrogenase 1, belongs to the aldehyde dehydrogenase enzyme family, which is involved in the metabolism of alcohol. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than othe
Revvity Health Sciences Inc HTRF Human Phospho-ULK1 (Ser758) Detection Kit, 500 Assay Points
HTRF Human Phospho-ULK1 (Ser758) Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter Human GPR32 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR32 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR32 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PTGER4 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human PTGER4 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure PTGER4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR2 (S167T) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)
The PathHunter Human AVPR2 (S167T) ENDO-EA Pharmacotrafficking Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure pharmacochaperone-based rescue of the mutant AVPR2's trafficking to early endosomes. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.